Establishment of human embryonic stem cell line Amicqui-2 using poor-quality embryos from Mexican population
نویسندگان
چکیده
منابع مشابه
Mesenchymal stem cells facilitate the derivation of human embryonic stem cells from cryopreserved poor-quality embryos.
BACKGROUND Human embryonic stem cells (hESCs) have opened up a new area of research in biomedicine. The efficiency of hESC derivation from frozen poor-quality embryos is low and normally achieved by plating embryos on mouse or human foreskin feeders (HFFs). We attempted to optimize embryo survival and hESC derivation. METHODS Three conditions were tested on frozen poor-quality embryos: (i) em...
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OBJECTIVE Human-embryonic-stem-cell (hESC) lines derived from chromosomally or genetically abnormal embryos obtained following preimplantation genetic diagnosis are valuable in investigating genetic disorders. MATERIALS AND METHODS In this study, a new hESC line, Center of Clinical Reproductive Medicine 8 (CCRM8) was established by isolation, culture, and passaging of the inner cell mass of m...
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embryonic stem cells (escs) are derived from the inner cell mass (icm) of blastocyst and differentiate into all three embryonic germ layers: ectoderm, endoderm, and mesoderm. in this study, escs are derived from hand made cloning (hmg) blastocysts and their efficiencies compared to escs derived from in vitro fertilization (ivf) embryos. feeder layer was used for escs culture, and culture medium...
متن کاملGeneration of cardiomyocytes from new human embryonic stem cell lines derived from poor-quality blastocysts.
Human embryonic stem (hES) cells represent a potential source for cell replacement therapy of many degenerative diseases. Most frequently, hES cell lines are derived from surplus embryos from assisted reproduction cycles, independent of their quality or morphology. Here, we show that hES cell lines can be obtained from poor-quality blastocysts with the same efficiency as that obtained from good...
متن کاملEstablishment of human embryonic stem cell lines from frozen-thawed blastocysts using STO cell feeder layers.
BACKGROUND Recently, human embryonic stem (hES) cells have become very important resources for basic research on cell replacement therapy and other medical applications. The purpose of this study was to test whether pluripotent hES cell lines could be successfully derived from frozen-thawed embryos that were destined to be discarded after 5 years in a routine human IVF-embryo transfer programme...
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ژورنال
عنوان ژورنال: Stem Cell Research
سال: 2019
ISSN: 1873-5061
DOI: 10.1016/j.scr.2018.101364